Annotated protein:Excitatory amino acid transporter 4 (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6). Gene symbol: SLC1A6. Taxonomy: Homo sapiens (Human). Uniprot ID: P48664
antibody wiki:
SynGO gene info:SynGO data @ SLC1A6
Ontology domain:Biological Process
SynGO term:neurotransmitter uptake (GO:0001504)
Annotated paper:Fairman WA, et al. "An excitatory amino-acid transporter with properties of a ligand-gated chloride channel" Nature. 1995 Jun 15;375(6532):599-603 PMID:7791878
Figure(s):Table 1
Annotation description:The paper charactrerizes EAAT4 as a L-glutamate and L-aspartate transporter that shuttles these across membranes in a sodium-dependent manner.

Table 1 summarized the results of an 3H-L-glutamate and 3H-L-aspartate uptake assays in Xenopus oocytes expressing the transporter. Injected oocytes had a 30-fold higher uptake of the compounds than uninjected controls.

rule: NAS_MFCC-BP
rationale:
Protein has "glutamate transmembrane transporter activity" and is localized in the presynaptic membrane (show with high-resolution technique). The inference to term 'neurotransmitter uptake (GO:0001504)' is trivial and non-controversial.
Pinf_CC:
[O35921] SynGO ID 771 (PMID:9570792, EM)
Evidence tracking, Biological System:Non-neuronal tissue
Evidence tracking, Protein Targeting:Over-expression
Evidence tracking, Experiment Assay:Transmembrane transport assay
Non-tracable Author Statement (NAS):This annotation required some inference over additional information/literature, and is not solely based on observations made in the annotated paper by its original authors.
Annotator(s):Noa Lipstein (ORCID:0000-0002-0755-5899)
Cordelia Imig (ORCID:0000-0001-7351-8706)
Vincent O'connor (ORCID:0000-0003-3185-5709)
Nils Brose (ORCID:0000-0003-0938-8534)
Lab:Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:792
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology