| Annotated protein: | Excitatory amino acid transporter 4 (High-affinity neuronal glutamate transporter) (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6). Gene symbol: SLC1A6. Taxonomy: Mus musculus (Mouse). Uniprot ID: O35544 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC1A6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | cochlea, glutamatergic |
| Annotated paper: | Dalet A, et al. "Glutamate transporters EAAT4 and EAAT5 are expressed in vestibular hair cells and calyx endings" PLoS One. 2012;7(9):e46261 PMID:23049999 |
| Figure(s): | Figure 5,6 and Table 1 |
| Annotation description: | The paper characterizes the expression of EAAT4 and EAAT5 in the vestibular type I hair cells. These cells have post-synaptic calycal endings which restrict the diffusion of glutamate following endocytosis, so that uptake of glutamate by supporting cells is not plausible. Figure 5: immunogold-labelling electron microscopy leads to the finding that the hair cells, as well as the calycal membrane facing the synaptic cleft, both harbor EAAT4 and EAAT5, supporting the notion that the transporters are located at both the pre- and postsynaptic membranes. Table 1 presents the quantification of the immuogold labeling. Figure 6 is a summarizing scheme. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 788 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |