| Annotated protein: | Sodium- and chloride-dependent creatine transporter 1 (CHOT1) (CT1) (Creatine transporter 1) (Solute carrier family 6 member 8). Gene symbol: SLC6A8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P28570 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A8 |
| Ontology domain: | Biological Process |
| SynGO term: | metabolism |
| Synapse type(s): | hippocampus |
| Annotated paper: | Dodd JR, et al. "Functional and immunocytochemical characterization of the creatine transporter in rat hippocampal neurons" J Neurochem. 2010 Nov;115(3):684-93 PMID:20731764 |
| Figure(s): | Fig. 5a+b |
| Annotation description: | The creatine transporter transports creatine in a sodium-dependent manner (Fig.5). Rat hippocampal neurons in culture are incubated with radiolabelled creatine for 15 min with or without sodium (Fig. 5a) indicating the requirements for sodium for the creatine transport into cells. The uptake of radiolabelled creatine is inhibited by creatine and creatine transporter competitive inhibitors (GPA, GBA, cycloCr, and GA, Fig. 5b). Samples of solubilized cells were taken for liquid scintillation counting protein determination. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | radioisotope assay evidence Transmembrane transport assay |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Review on creatine/creatine transporter function. @ PMID:18652074 |
| SynGO annotation ID: | 775 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |