| Annotated protein: | Excitatory amino acid transporter 4 (High-affinity neuronal glutamate transporter) (Sodium-dependent glutamate/aspartate transporter) (Solute carrier family 1 member 6). Gene symbol: SLC1A6. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O35921 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC1A6 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Dehnes Y, et al. "The glutamate transporter EAAT4 in rat cerebellar Purkinje cells: a glutamate-gated chloride channel concentrated near the synapse in parts of the dendritic membrane facing astroglia" J Neurosci. 1998 May 15;18(10):3606-19 PMID:9570792 |
| Figure(s): | Figure 6, 7, 8 |
| Annotation description: | The paper uses pre-embedding immuno-EM. Figure 1 demonstrates the specificity of a newly made antibody against human EAAT4 in this paper (anti-D537). In figure 6, pre-embedding electron microscopic immunocytochemistry using peroxidase-based labelling shows specific staining in spines, but no staining in axons or in glial processes. Figure 7: postembedding immunogold EM of EAAT4 demonstrating labelling in membranes of Purkinje cell spines, spine necks, and dendrites. Figure 8 illustrates that in the vast majority of cases the immunolabelling is seen in contact sites with astroglia and not in contact sites with the postsynaptic membrane, or in the parts of the spine membrane that are in contact with presynaptic nerve terminals. Figure 11b - quantification of EAAT4 immunoreactivity along different parts of the Purkinje cell membrane, demonstrating that the majority of particles are found in contact sites to astroglia in dendrite (11b column b) or in spines (11b column f) |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | The paper characterizes the expression of EAAT4 and EAAT5 in the vestibular type I hair cells. These cells have post-synaptic calycal endings which restrict the diffusion of glutamate following endocytosis, so that uptake of glutamate by supporting cells is not plausible. Figure 5: immunogold-labelling electron microscopy leads to the finding that the hair cells, as well as the calycal membrane facing the synaptic cleft, both harbor EAAT4 and EAAT5, supporting the notion that the transporters are located at both the pre- and postsynaptic membranes. Table 1 presents the quantification of the immuogold labeling. Figure 6 is a summarizing scheme. @ PMID:23049999 |
| SynGO annotation ID: | 771 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |