| Annotated protein: | Abl interactor 1 (Abelson interactor 1) (Abi-1) (Eps8 SH3 domain-binding protein) (Eps8-binding protein) (e3B1). Gene symbol: ABI1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9QZM5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABI1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | brain, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Proepper C, et al. "Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation" EMBO J. 2007 Mar 7;26(5):1397-409 PMID:17304222 |
| Figure(s): | Fig 1f, Fig 2 |
| Annotation description: | Authors study the localization of Abi-1 in rat hippocampal cultures and rat brain using a monoclonal antibody against Abi-1. Immunoelectron microscopy revealed Abi-1 at postsynaptic densities. In line with this, immunostaining of Abi-1 showed colocalization of the protein with Shank3, a postsynaptic excitatory marker, while coimmunostaining with the presynaptic marker bassoon revealed a juxtaposed pattern, in agreement with the postsynaptic localization of Abi-1. Subcellular fractionation revealed enrichment of Abi-1 in PSD fractions. 14/3/2017 Pim - Abi1 shown to interact directly with Shank3 but not with Shank1 (Fig.1). Shank3 is a typical PSD protein. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Western blot Biochemical fractionation (generic) Protein-protein interaction (generic) |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 770 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |