| Annotated protein: | Sodium- and chloride-dependent GABA transporter 3 (GAT-3) (Solute carrier family 6 member 11). Gene symbol: SLC6A11. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P31647 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A11 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Melone M, et al. "A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex" Brain Struct Funct. 2015 Mar;220(2):885-97 PMID:24368619 |
| Figure(s): | Figure 4a-g, Figure 5f-I, and Table 1 |
| Annotation description: | The paper quantitatively describes the distribution of GAT-1 and GAT-3 at the rat cortex using pre-embedding peroxidase labeling and postimbedding immunogold labeling. Figure 3: electron microscopy images of peroxidase-based immunoreactivity in preembedded material of cortical layers 2-3. In figure 3e axonal labeling is evident. Figure 3H quantifies the fraction of profiles where immunoreactivity was observed in axons or dendrites. Figure 4h-n expands this analysis . In h-m example images of immunoreactivity in axon terminals, perisynaptic astrocytic processes and post-synaptic elements are shown. In figure 4n a quantification of fraction of profiles where immunoreactivity was observed in the above-mentioned cellular compartments, with a further division into symmetric and asymmetric synapses. Table 1 presents the density of GAT-3 gold particles (following post-embedding immunogold EM) associated with the plasma membrane of axon terminals of symmetric synapses. Here, a quantification of the background is also given (immunogold labeling of the nuclear compartment), to demonstrate specificity of the labeling. Figure 5f-i present the analysis of post-embedding immunogold EM profiles where membrane-associated particles were visualized, and quantifies the distance from the active-zone. Taken together, the data supports a presynaptic localization of GAT-3 at the plasma membrane and membranous cellular compartments in the pre synapse. 1/7/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 768 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |