| Annotated protein: | Sodium- and chloride-dependent GABA transporter 1 (GAT-1) (Solute carrier family 6 member 1). Gene symbol: SLC6A1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P23978 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Melone M, et al. "A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex" Brain Struct Funct. 2015 Mar;220(2):885-97 PMID:24368619 |
| Figure(s): | Figure 2, Figure 4a-g |
| Annotation description: | The paper quantitatively describes the distribution of GAT-1 and GAT-3 at the rat cortex using pre-embedding peroxidase labeling and postimbedding immunogold labeling. Figure 2: electron microscopy images of peroxidase-based immunoreactivity in preembedded material of cortical layers 2-3. In figure 2e dendritic labeling is evident. Figure 2I quantifies the fraction of profiles where immunoreactivity was observed in axons or dendrites. Figure 4a-g expands this analysis . In 4D,E example images of immunoreactivity in axon terminals, perisynaptic astrocytic processes and post-synaptic elements are shown. In figure 4g a quantification of fraction of profiles where immunoreactivity was observed in the above-mentioned cellular compartments, with a further division into symmetric and asymmetric synapses. Taken together, the data supports a postsynaptic localization of GAT-1. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 767 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |