| Annotated protein: | Leucine-rich repeat transmembrane neuronal protein 2 (Leucine-rich repeat neuronal 2 protein). Gene symbol: LRRTM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D4A7P2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRRTM2 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic density assembly (GO:0099151) |
| Synapse type(s): | hippocampus, glutamatergic brain Schaffer collateral synapse (CA3->CA1) Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Linhoff MW, et al. "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers" Neuron. 2009 Mar 12;61(5):734-49 PMID:19285470 |
| Figure(s): | Fig.6 |
| Annotation description: | Fig. 2; LRRTM2 has a pre-synaptogenic activity when transfected in COS cells cocultured with hippocampal neurons Literal: "we measured the amount of synapsin clustering associated with transfected COS cells and not associated with MAP2-positive dendrites to exclude interneuronal synapses. Robust synaptogenic activity was observed for LRRTM2-CFP" 24/5/2017 Pim - Fig.3: non-neuronal cells transfected with LRRTM2-CFP (rat protein) and NMDAR (simulating a postsynaptic neuron) are able to induce presynaptic differentiation of a neuron (detectable glutamate release from SV's) Fig. 4; clustering of synapsin was induced by beads coated with LRRTM2 LRR-AP Literal: "LRRTM2 LRR-AP, was linked via biotinylated anti-myc antibody onto neutravidin beads. Contact of these LRR-APcoated beads with isolated axons of hippocampal neurons induced clustering of synapsin or VGLUT1 at contact sites (Figures 4A and 4B, right panels). Beads coated with the control AP protein did not display synaptogenic activity (Figures 4A and 4B, left panels). Random counts revealed synapsin clustering at 34.1% of LRR-AP bead contacts and only 2.1% of control AP bead contacts (n = 50 fields). The mean synapsin intensity under all LRR-AP beads was 6.3-fold higher than that under control AP beads (p < 0.0001). Clustering of the active zone marker bassoon could also be detected at LRR-AP bead-axon contact sites. In fact, there appeared to be separation between the bassoon-labeled active zone and the VGLUT1-positive vesicle pool, with the active zone most closely apposed to the bead surface (Figure 4B, right panel, inset..." |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Electrophysiology (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 761 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |