| Annotated protein: | CREB-regulated transcription coactivator 1 (Mucoepidermoid carcinoma translocated protein 1 homolog) (Transducer of regulated cAMP response element-binding protein 1) (TORC-1) (Transducer of CREB protein 1). Gene symbol: CRTC1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q157S1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CRTC1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ch'ng TH, et al. "Activity-dependent transport of the transcriptional coactivator CRTC1 from synapse to nucleus" Cell. 2012 Jul 6;150(1):207-21 PMID:22770221 |
| Figure(s): | Fig S1a,b,d,e; Fig1b,c,d,e,f,g |
| Annotation description: | Authors used an antibody that specifically recognizes CRTC1 but not CRTC2 to study the localization of CRTC1 using spinning disk confocal microscopy. CRTC1 immunoreactivity was found in dendrites and spines, with 99% of PSD95-positive puncta containing CRTC1. This colocalization is activity regulated. Accordingly, CRTC1 was found in synaptoneurosome and PSD fractions. Pull-down experiments using recombinant proteins show that CRTC1 binds to 14-3-3 epsilon, a protein found in dendritic spines, suggesting that the synaptic tethering of CRTC1 relies on the interaction with 14-3-3 epsilon. This interaction is also regulated by activity. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) Protein-protein interaction (generic) |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 758 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |