| Annotated protein: | Ankyrin repeat and sterile alpha motif domain-containing protein 1B (Amyloid-beta protein intracellular domain-associated protein 1) (AIDA-1) (E2A-PBX1-associated protein) (EB-1). Gene symbol: ANKS1B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P0C6S7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ANKS1B |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Jacob AL, et al. "Organization of amyloid-beta protein precursor intracellular domain-associated protein-1 in the rat brain" J Comp Neurol. 2010 Aug 15;518(16):3221-36 PMID:20575057 |
| Figure(s): | Fig 1a, Fig.9, Fig12 |
| Annotation description: | Authors used a polyclonal antibody against AIDA-1 generated in rabbit to study the protein distribution in vivo. This antibody identifies at least different isoforms of AIDA-1 (AIDA-1d, AIDA-1e and EB-1), being AIDA-1d and 1e found in synaptosomes and PSD fractions. Confocal imaging revealed a prominent staining of AIDA1 in the stratum lucidum of CA3 which colocalized with VGLUT1 staining, suggesting the localization of AIDA1 in mossy fiber terminals. This was confirmed by subsequent preembedding immunoperoxidase experiments that showed immunolabeling of AIDA1 in mossy fiber terminals, concentrated at the center of synaptic vesicle pools. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 756 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |