| Annotated protein: | Ankyrin repeat and sterile alpha motif domain-containing protein 1B (Amyloid-beta protein intracellular domain-associated protein 1) (AIDA-1) (E2A-PBX1-associated protein) (EB-1). Gene symbol: ANKS1B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P0C6S7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ANKS1B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Jacob AL, et al. "Organization of amyloid-beta protein precursor intracellular domain-associated protein-1 in the rat brain" J Comp Neurol. 2010 Aug 15;518(16):3221-36 PMID:20575057 |
| Figure(s): | Fig 1a, Fig 13 |
| Annotation description: | Authors used a polyclonal antibody against AIDA-1 generated in rabbit to study the protein distribution in vivo. This antibody identifies at least different isoforms of AIDA-1 (AIDA-1d, AIDA-1e and EB-1), being AIDA-1d and 1e found in synaptosomes and PSD fractions. Immunogold EM performed in hippocampus and more precisely in CA1 stratum radiatum shows gold particles closely associated with the PSD. Analysis of the location of those particles reveals a mean distance of 13 +/- 2 nm from the postsynaptic plasma membrane. 14/6/2017 Pim - Literal: "AIDA-1 is distributed fairly uniformly along the PSD, decreasing rapidly at its edge" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 755 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |