| Annotated protein: | Sodium- and chloride-dependent glycine transporter 1 (GlyT-1) (GlyT1) (Solute carrier family 6 member 9). Gene symbol: SLC6A9. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P28572 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SLC6A9 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic vesicle membrane (GO:0030285) |
| Synapse type(s): | brain |
| Annotated paper: | Cubelos B, et al. "Localization of the glycine transporter GLYT1 in glutamatergic synaptic vesicles" Neurochem Int. 2014 Jul;73:204-10 PMID:24036061 |
| Figure(s): | Figure 2 and figure 4 |
| Annotation description: | Figure 2a: In the synaptic vesicle fraction obtained during the subcellular fractionation of rat brain tissue GlyT1, VGLUT1 and synaptophysin expression can be detected by western blotting. Figure 2b: in fractions isolated from sucrose gradient sedimentation of the LP2 fraction prepared for figure 2a, GlyT1, VGLUT1 and synaptophysin expression can be detected in overlapping fractions by western blotting. Figure 4: immunogold-EM of Sucrose gradient purified LP2 fractions number 5-8 demonstrating that GlyT1 can be found at the membrane of vesicles in this fraction (4a). Colocalization of GlyT1 with synaptophysin (4b) or with VGLUT1 (4c) at the same membrane compartment demonstrated by dual immunogold-EM. No knockout controls, antibody source is not mentioned. 1/7/2017 Pim - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Immunohistochemical analysis of GlyT1 expression in neurons. Figure 6 A-F: Pre-embedding immunogold staining of GLYT1 in the hippocampus the neocortex, and the spinal cord. Figure 6 G-I, K: Post-embedding immunogold of GLYT1 in the dentate gyrus and the molecular layer of the cerebellum. The profiles show localization in both the pre- and postsynaptic compartments, as well as in glial processes. @ PMID:15749988 |
| SynGO annotation ID: | 747 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |