| Annotated protein: | Leucine-rich repeat transmembrane neuronal protein 1. Gene symbol: LRRTM1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D4A6D8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRRTM1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Linhoff MW, et al. "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers" Neuron. 2009 Mar 12;61(5):734-49 PMID:19285470 |
| Figure(s): | Fig. 5a |
| Annotation description: | Fig. 5a shows that trsnsfected YFP-LRRTM1 colocalize with VGLUT1 and PSD-95 Literal: "To begin to assess subcellular localization, we created extracellular YFP fusions of LRRTM1 and LRRTM2 and transfected hippocampal neurons in culture. YFP-LRRTM-1 or -2 expressed at low level in hippocampal neurons traffic to dendrites and assume a punctate, synaptic-like pattern (Figures 5A-5C). YFP-LRRTM-1 or -2 colocalized with PSD-95 family proteins opposite VGLUT1 puncta. YFP-LRRTM-1 and -2 appeared to be exclusively colocalized with the excitatory postsynaptic scaffolding proteins of the PSD-95 family but not with the inhibitory postsynaptic scaffolding protein gephyrin." 8/6/2017 Pim - Localization of LRRTM1 to the postsynaptic membrane is derived from (i) colocalization with PSD-95 and (ii) the TM domains of the proteins, (iii) the compartment of related protein LRRTM2 (Fig.7), and, most importantly, the (iii) synaptogenic ability of the protein when expressed on the plasma membrane of non-neuronal cells. It must be noted that in this publication and in others (PMID:17667961) the protein is very inefficiently transported to the plasma membrane. Literal: "Although the most synaptogenic factors tended to have increased axon contact area, the same phenomenon did not hold true for LRRTM1-CFP. This result is likely due to the reduced surface expression of LRRTM1 relative to the other family members. We observed that LRRTM1 accumulates extensively in the endoplasmic reticulum of transfected nonneuronal cells (data not shown), and this result has been observed previously (Francks et al., 2007)." - I agree with the experts to not choose a more specific term "postsynaptic density memrane" because of the sub-resolution data. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 737 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |