| Annotated protein: | Leucine-rich repeat transmembrane neuronal protein 3. Gene symbol: LRRTM3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BZ81 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRRTM3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | brain, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Um JW, et al. "LRRTM3 Regulates Excitatory Synapse Development through Alternative Splicing and Neurexin Binding" Cell Rep. 2016 Feb 2;14(4):808-822 PMID:26776509 |
| Figure(s): | Fig. S2e,f,g,h,i,k |
| Annotation description: | Fig. S2e; biochemical fractionation showe that LRRTM3 is enriched in the PSD fraction Fig. S2f,g,h,i; endogenous LRRTM3 colocalizes with PSD-95 and VGLUT1 but not with Gephyrin and GAD67 in hippocampal cultures Fig. S2k; in hippocampal cultured neurons overexpressed Venus-LRRTM3 colocalize with VGLUT1 and PSD-95. Literal: "Notably, L3L was enriched in detergent-resistant PSD fractions (Figure S2E). These data indicate that LRRTM3 (at least L3L) is associated with excitatory synaptic membranes. In accord with biochemical data, confocal microscopy revealed that L3L immunoreactivity was closely associated with the excitatory marker proteins VGLUT1 and PSD-95, but not with the inhibitory marker proteins GAD67 and gephyrin in cultured hippocampal neurons (Figures S2F-S2J). L3L immunoreactivity was also prominent outside VGLUT1- or PSD-95-positive excitatory synaptic sites, consistent with its widespread distribution in various subcellular fractions (see Figure S2D). To corroborate these observations, we expressed monomeric Venus (mVenus) fluorescent protein fused to L3L (L3L-mVenus) in cultured hippocampal neurons and immunostained them for VGLUT1 and PSD-95 (Figure S2K). mVenus immunofluorescence in L3L-mVenus-expressing neurons was clustered at excitatory synapse sites (55% ± 5% and 49% ± 6% of L3L-mVenus-positive puncta matches with PSD-95 and VGLUT1, respectively), suggesting that endogenous L3L proteins, similar to L3S, localize to excitatory synapses" 8/6/2017 Pim - LRRTM3 has TM domain --> "integral component ...." - Localization to the postsynaptic density membrane is supported (not demonstrated) by the PPI with PSD-95 protein. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 736 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |