| Annotated protein: | Protein kinase C and casein kinase substrate in neurons protein 1 (Syndapin-1). Gene symbol: PACSIN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61644 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PACSIN1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Koch D, et al. "Proper synaptic vesicle formation and neuronal network activity critically rely on syndapin I" EMBO J. 2011 Sep 16;30(24):4955-69 PMID:21926968 |
| Figure(s): | fig 3, fig 4, fig 7 |
| Annotation description: | The authors generate a syndapin 1 KO mouse. In figure 3 they use FM1-43 uptake assays to measure endocytosis in cultured hippocampal neurons. At low frequency stimulation there was a very small decrease in FM1-43 dye uptake but at higher frequency stimulation there was larger relative decrease in SV endocytosis when comparing KO to wt. In figure 4 they use EM in the mouse retina. This synapse fires extensively in the dark. Shifting to light causes a strong compensatory endocytosis. The synapses from KO mice showed numerous irregularities when shifting to light including accumulation of clathrin-coated pits at the plasma membrane and alterations in SV size. In figure 7, the authors use whole-cell recordings from CA1 pyramidal neurons of syndapin I KO mice and observe reduced frequencies of miniature excitatory and inhibitory potentials consistent with a defect in synaptic vesicle recycling in syndapin I KO neurons. 19/6/2017 Pim - Literal: "Detailed molecular analyses demonstrate that syndapin I plays an important role in the recruitment of all dynamin isoforms, central players in vesicle fission reactions, to the membrane." Since syndapin seems involved in the recruitment of dynamin I do no classify this protein as a "regulator of..." SVE. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Electrophysiology (generic) Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 735 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |