| Annotated protein: | Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (PIP5K1-gamma) (PtdIns(4)P-5-kinase 1 gamma) (EC 2.7.1.68) (Phosphatidylinositol 4-phosphate 5-kinase type I gamma) (PIP5KIgamma). Gene symbol: PIP5K1C. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5I6B8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIP5K1C |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic endocytic zone membrane (GO:0098835) |
| Synapse type(s): | brainstem brain |
| Annotated paper: | Wenk MR, et al. "PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse" Neuron. 2001 Oct 11;32(1):79-88 PMID:11604140 |
| Figure(s): | fig 2, fig 6 |
| Annotation description: | Fig 2 uses immunofluorescence analysis to demonstrate that PIP kinase Igamma is localized in the presynaptic compartment. Fig 6 uses immunogold electron microscopy to demonstrate that PIP kinase Igamma is localized to the presynaptic plasma membrane as well as the cytososl and coated endocytic intermediates. In figure 6C, the authors report a strikingly selective localization of PIPKIγ immunoreactivity on membrane regions directly adjacent to clathrin-coated buds while synaptic vesicles were consistently unlabelled. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 734 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |