| Annotated protein: | Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (PIP5K1-gamma) (PtdIns(4)P-5-kinase 1 gamma) (EC 2.7.1.68) (Phosphatidylinositol 4-phosphate 5-kinase type I gamma) (PIP5KIgamma). Gene symbol: PIP5K1C. Taxonomy: Mus musculus (Mouse). Uniprot ID: O70161 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIP5K1C |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle endocytosis (GO:1900242) |
| Synapse type(s): | neocortex |
| Annotated paper: | Di Paolo G, et al. "Impaired PtdIns(4,5)P2 synthesis in nerve terminals produces defects in synaptic vesicle trafficking" Nature. 2004 Sep 23;431(7007):415-22 PMID:15386003 |
| Figure(s): | fig 4, fig 3, fig 5 |
| Annotation description: | In fig 4b the authors use cortical cultures from control and knockout mice. Using transfected syaptopHlourin they demonstrated that endocytosis, as determined by quenching of pHlourin fluorescence is decreased in KO mice compared to control following a 10 Hz for 30 sec stimulus. In fig 4d they use the same experimental set up to demonstrate that endocytosis is decreased in the KO mice during stimulation. In fig 3C,D the authors demonstrate that synapses in the KO mice depress faster in response to a 20 Hz train of 1000 action potentials. fig 3E reveals that the readily-releasable pool of synaptic vesicles is smaller in the KO mice. In fig 5C, the increase in CCVs observed following stimulation of cortical neurons with KCl (driving synaptic vesicle release) is less in KO neurons than in control neurons. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Electrophysiology (generic) Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| Additional literature: | See also this paper. @ PMID:17290217 |
| SynGO annotation ID: | 733 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |