| Annotated protein: | Beta-soluble NSF attachment protein (SNAP-beta) (N-ethylmaleimide-sensitive factor attachment protein beta). Gene symbol: NAPB. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P85969 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NAPB |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | brain |
| Annotated paper: | Nishiki T, et al. "Distribution of soluble N-ethylmaleimide fusion protein attachment proteins (SNAPs) in the rat nervous system" Neuroscience. 2001;107(3):363-71 PMID:11718992 |
| Figure(s): | Fig.3, Fig.4B,D,F,H, Fig.5B,C, Fig.6C,D |
| Annotation description: | SNAP= soluble N-ethylmaleimide fusion protein attachment protein. Subcellular fractionation and Western Blot with an isoform-specific antibody against beta-SNAP indicates that beta-SNAP is present in membrane and soluble fractions of synaptosomes (Fig.3). Immunohistochemical analysis indicates the presence of betaSNAP predominantly in synapse-rich regions (e.g. stratum radiatum in the hippocampus, Fig. 4). Pre-embedding electron microscopy of tissue sections and crude synaptic membranes indicates the presence of beta-SNAP in presynaptic plasma membranes, but also postsynaptic densities (Fig. 5+6). Isoform-specific anti-SNAP antibodies were raised to the sequence: C)SIQGDGEGDGDLK (anti-β-SNAP, residues 286-298 of β-SNAP). Immunoblotting analysis demonstrates the specificity of the antibody (Fig.1) 6/6/2017 Pim - More specific annotation to f.i. "postsynaptic density" discussed in similar instances but disregarded by Brose lab. Annotation to "postynapse" is allowed therefore. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Microscopy (generic) Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 726 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |