| Annotated protein: | Interleukin-1 receptor accessory protein (IL-1 receptor accessory protein) (IL-1RAcP) (EC 3.2.2.6) (Interleukin-33 receptot beta chain). Gene symbol: IL1RAP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61730-1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ IL1RAP |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of presynapse assembly (GO:1905606) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Yoshida T, et al. "Interleukin-1 receptor accessory protein organizes neuronal synaptogenesis as a cell adhesion molecule" J Neurosci. 2012 Feb 22;32(8):2588-600 PMID:22357843 |
| Figure(s): | Fig. 1, 2, 3, 4, 8 |
| Annotation description: | Fig. 1; IL-1RAcP induces presynaptic differentiation when expressed in HEK293T co-cultured with cortical neurons. Literal: "HEK293T cells were transfected with EGFP and respective IL-1R family proteins tagged with FLAG epitope at the N terminus and were seeded on the top of cultured cortical neurons."we detected robust staining signals for Bassoon on the axons in contact with HEK293T cells expressing FLAG-IL-1RAcP Fig.2 Beads with Fc-fused IL-1RAcP ECD induce presynaptic differentiation in co-cultured neurons Fig. 3d,e,f ; siRNA-mediated knockdown of IL-1RAcP isoforms in cortical neurons reduced the staining signals for presynaptic Bassoon and decreased the number of dendritic spines. Literal: "The siRNA against Il1rap was transfected into cultured cortical neurons at DIV 10. After 4d of incubation, we observed a significant reduction of staining signals for presynaptic Bassoon along the dendrites of the transfected cortical neurons and a decrease of number of dendritic protrusions (Fig. 3D-F)" Fig. 4a,b; transfected FLAG-IL-1RAcP in cortical neurons increased punctate immunostaining signals for Bassoon Literal: "Transfection of cultured cortical neurons at DIV 12 with the expression vector for FLAG-IL-1RAcP increased punctate immunostaining signals for Bassoon but exerted little effect on dendritic protrusions (Fig. 4) Fig.8 IL-1RAcPb and IL-1RAcP (not shown) requires PTPδ for presynaptic differentiation |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 715 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |