| Annotated protein: | General receptor for phosphoinositides 1-associated scaffold protein (GRP1-associated scaffold protein) (95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein) (PSD-95 PDZ domain-containing protein) (Tamalin). Gene symbol: TAMALIN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8R4T5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TAMALIN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | brain, glutamatergic |
| Annotated paper: | Kitano J, et al. "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins" J Neurosci. 2002 Feb 15;22(4):1280-9 PMID:11850456 |
| Figure(s): | Fig. 5/6 |
| Annotation description: | Fig.5 "The results indicated that tamalin mainly forms a complex with group 1 mGluRs in the brain" Fig. 6 Tamalin is enriched in PSD III fraction. Literal "We examined the postsynaptic membrane localization of tamalin and cytohesin-2 by immunoblotting of serially extracted synaptosomal membrane proteins (Fig. 6A). Both mGluR1a and PSD-95 were mostly concentrated at the PSD III fraction, whereas the presynaptic marker protein, synaptophysin, was deprived from synaptosome by detergent extraction. Both tamalin and cytohesin-2 were enriched up to the PSD II fraction but slightly and completely deprived from the PSD III fraction, respectively. The results indicated that both tamalin and cytohesin-2 are localized at postsynaptic membrane fractions and are relatively tightly and loosely associated with postsynaptic membranes, respectively." 20/9/2017 Pim - Authors implie that mGlur1a is predominantly localized to the postsynaptic membrane and can be considered a marker of the PSD membrane. Literal: "mGluR1a is mostly localized at the postsynaptic membrane in neuronal cells (Martin et al., 1992)." For this reason I have added PPI to the evidence section. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| Additional literature: | Fig. 3. GRASP/Tamalin coimmunoprecipitate with PSD-95 and other synaptic scafoold proteins. Literal: "S-SCAM, PSD-95, SAPAP1 were coimmunoprecipitated from rat brain membrane fractions with anti-tamalin antibody (Fig. 3A). In addition, Mint2 and CASK were immunoprecipitated, but neither Mint1 nor Veli1/2/3 was precipitated with anti-tamalin antibody (Fig. 3B)." @ PMID:12586822 |
| SynGO annotation ID: | 714 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |