| Annotated protein: | Neuronal calcium sensor 1 (NCS-1) (Frequenin homolog) (Frequenin-like protein) (Frequenin-like ubiquitous protein). Gene symbol: NCS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62168 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NCS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Tsujimoto T, et al. "Neuronal calcium sensor 1 and activity-dependent facilitation of P/Q-type calcium currents at presynaptic nerve terminals" Science. 2002 Mar 22;295(5563):2276-9 PMID:11910115 |
| Figure(s): | Figure 3a,b |
| Annotation description: | NCS-1 immunoreactivity at the Calyx of Held synapse. Figure 3a shows a prominent immunofluorescence signal for NCS-1 at the postsynaptic compartment of MNTB cell soma (postsynaptic compartments of the calyx of Held synapses), that does not colocalizes with staining against the presynaptic marker Syntaxin (it is not mentioned which isoform). Figure 3b evaluates the antibody specificity (since a KO control is missing): Pretreatment of the NCS-1 antibody with recombinant NCS-1 leads to no NCS-1 immunofluorescence, but has no effect on the syntaxin signal. 30/5/2017 Pim: - Literal: " NCS-1 immunofluorescence signal (green, left) and syntaxin immunofluorescence signal (red, center) partially overlapped (yellow, right), indicating that NCS-1 is expressed in both the presynaptic and postsynaptic compartments." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 708 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |