Annotated protein:Neuronal calcium sensor 1 (NCS-1) (Frequenin homolog) (Frequenin-like protein) (Frequenin-like ubiquitous protein). Gene symbol: NCS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62168
antibody wiki:
SynGO gene info:SynGO data @ NCS1
Ontology domain:Cellular Component
SynGO term:presynaptic cytosol (GO:0099523)
Synapse type(s):Calyx of Held
Annotated paper:Tsujimoto T, et al. "Neuronal calcium sensor 1 and activity-dependent facilitation of P/Q-type calcium currents at presynaptic nerve terminals" Science. 2002 Mar 22;295(5563):2276-9 PMID:11910115
Figure(s):Figure 3a,b
Annotation description:NCS-1 immunoreactivity at the Calyx of Held synapse.

Figure 3a shows a prominent immunofluorescence signal for NCS-1 at the presynaptic compartment for calyx of Held synapses, that partially colocalizes with staining against the presynaptic marker Syntaxin (it is not mentioned which isoform).
Figure 3b evaluates the antibody specificity (since a KO control is missing): Pretreatment of the NCS-1 antibody with recombinant NCS-1 leads to no NCS-1 immunofluorescence, but has no effect on the syntaxin signal.

30/5/2017 Pim:
- Literal: " NCS-1 immunofluorescence signal (green, left) and syntaxin immunofluorescence signal (red, center) partially overlapped (yellow, right), indicating that NCS-1 is expressed in both the presynaptic and postsynaptic compartments."
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Microscopy (generic)
Annotator(s):Noa Lipstein (ORCID:0000-0002-0755-5899)
Cordelia Imig (ORCID:0000-0001-7351-8706)
Vincent O'connor (ORCID:0000-0003-3185-5709)
Nils Brose (ORCID:0000-0003-0938-8534)
Lab:Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany
SynGO annotation ID:707
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology