Annotated protein:Amphiphysin. Gene symbol: AMPH. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q7TQF7
antibody wiki:
SynGO gene info:SynGO data @ AMPH
Ontology domain:Biological Process
SynGO term:synaptic vesicle endocytosis (GO:0048488)
Synapse type(s):neocortex
Annotated paper:Di Paolo G, et al. "Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice" Neuron. 2002 Feb 28;33(5):789-804 PMID:11879655
Figure(s):fig 4, fig 5
Annotation description:The authors generate an amphiphysin I KO mouse. In fig3 they demonstrate that uptake of the endocytic tracer FM2-10 is reduced in synaptosomes prepared from KO mice when compared to wild-type mice.

In fig 5 the authors use EM to demonstrate that loading of synaptic vesicles with a fluid phase tracer, HRP is decreased in cortical neurons prepared from amphiphysin KO mice.
Evidence tracking, Biological System:Intact tissue
Cultured neurons
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:Electron Microscopy
Optical physiology
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
SynGO annotation ID:705
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology