| Annotated protein: | Phosphatidylinositol-binding clathrin assembly protein (Clathrin assembly lymphoid myeloid leukemia protein) (rCALM). Gene symbol: PICALM. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O55012 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PICALM |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Koo SJ, et al. "SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors clathrin assembly lymphoid myeloid leukemia (CALM) and AP180 at synapses" Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13540-5 PMID:21808019 |
| Figure(s): | fig 1 |
| Annotation description: | The authors use siRNA to knock down CALM in cultured rat hippocampal neurons. Using synaptobrevin pHlourin they find a defect in internalization of synaptobrevin. However, there is no defect in internalization of vGlut pHlourin. They thus argue that CALM is not involved in synaptic vesicle endocytosis per se but in the sorting of synaptobrevin to synaptic vesicles. 29/5/2017 Pim - From Uniprot: "Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly" PICALM BP is part of the endocytosis process -> "regulates ..." box is not checked. - Synaptobrevin is a marker protein of SV's |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 704 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |