| Annotated protein: | Disintegrin and metalloproteinase domain-containing protein 22 (ADAM 22). Gene symbol: ADAM22. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9R1V6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADAM22 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645) |
| Synapse type(s): | brain, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Fukata Y, et al. "Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission" Science. 2006 Sep 22;313(5794):1792-5 PMID:16990550 |
| Figure(s): | Fig. 4 |
| Annotation description: | Fig. 4 ADAM22 binds LGl1 and regulates the number of synaptic AMPARs Literal "The effects of LGI1 on synaptic currents could be prevented by preincubation of LGI1 with ADAM22-ED (Fig. 4A) (ADAM22-ED 0 0.59 T 0.06; LGI1þADAM22-ED 0 0.65 T 0.07), suggesting that an interaction between LGI1 and ADAM22 was required for the increased AMPA/NMDA ratio. To determine whether LGI1 directly affects the number of synaptic AMPARs, we measured AMPAR-mediated spontaneous miniature excitatory postsynaptic currents (mEPSCs). LGI1 incubation increased the average amplitude of these events (n 0 17, both groups, P G 0.005), and preincubation of LGI1 with ADAM22-ED blocked this increase (Fig. 4, B and D)." 7/6/2017 Pim - The role of ADAM22 in this process is derived form combined evidences: (i) ADAM22 is a receptor for LGI1 in neurons (Fig.3). Literal: " Taken together, these results imply that secreted LGI1 serves as a specific extracellular ligand for ADAM22 and that the LGI1/ADAM22 complex is scaffolded by PSD-95." (ii) ADAM22-ECD infusion acts as a dom.neg. for the activation of postsynaptic ADAM22 receptors by LGI1 (Fig.4) that mediates the increase of synaptic AMPA receptors. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 703 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |