| Annotated protein: | Synaptojanin-1 (EC 3.1.3.36) (Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1). Gene symbol: SYNJ1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8CHC4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SYNJ1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle uncoating (GO:0016191) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Cremona O, et al. "Essential role of phosphoinositide metabolism in synaptic vesicle recycling" Cell. 1999 Oct 15;99(2):179-88 PMID:10535736 |
| Figure(s): | fig 3, fig 7c |
| Annotation description: | The authors generate synaptojanin 1 knock out (KO) mice. Previous studies had established that synaptojanin is a lipid phosphatase dephosphorylating PtdIns(4,5)P2 to PtdIns(4)P. PtdIns(4,5)P2 interacts with numerous components required for coating of clathrin coated vesicles (CCVs). The KO mice display an accumulation of CCVs in the presynaptic nerve terminal (fig 3) and recovery from synaptic depression following a strong stimulation (10Hz for 200 sec), an indication of SV recycling is strongly reduced in the KO mice. 19/6/2017 Pim - Justifcation for selected term by expert: "When synaptojanin is null the presynaptic nerve terminal accumulates CCVs (fig 3A/C/D). This not seen in the wt animals (fig 3B) and in fact one almost never observes CCVs in nerve terminals under wt conditions. This is because the clathrin coats are rapidly removed. This observation is entirely consistent with the fact that synaptojanin dephosphorylates PtdIns(4,5)P2 and this PtdIns species is required for the clathrin coating of CCVs. The inference is that if the CCVs can not uncoat, then the SVs can not rejoin the SV functional pool." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Whole-cell patch clamp |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 701 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |