| Annotated protein: | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (EC 5.2.1.8) (Peptidyl-prolyl cis-trans isomerase Pin1) (PPIase Pin1). Gene symbol: PIN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9QUR7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PIN1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at postsynapse (GO:0140252) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Xu L, et al. "Pathological Role of Peptidyl-Prolyl Isomerase Pin1 in the Disruption of Synaptic Plasticity in Alzheimer's Disease" Neural Plast. 2017;2017:3270725 PMID:28458925 |
| Figure(s): | Fig.4, 5, 6 |
| Annotation description: | Background: Pin 1, or peptidyl-prolyl cis/trans isomerase (PPIase), isomerizes phospho-Serine/Threonine-Proline motifs. It does not act on protein ubiquitination, but may do so in an indirect manner via changes in protein conformation that makes Ubi-sites available for Ubi-ligases. Fig.4: PIN1 co-precipitates with Shank3 from synaptosomal preps. KD of PIN1 results in increased Shank3 ubiquitination (mouse, C57/BL6 cortical neurons at 21 DIV, shRNA) Fig.5 KD of PIN1 results in loss of Shank3 from synapses (mouse, C57/BL6 cortical neurons at 21 DIV, shRNA) Fig.6 Inhibition of PIN1 (PiB antagonist) changes the protein turnover of Shank3 and PSD-95 upon NMDAR stimulation (synaptic receptors). This is measured in cultured neurons, not synapses specifically; the NMDAR-stimulation is synaptic though. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 697 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |