| Annotated protein: | Tripartite motif-containing protein 3 (EC 2.3.2.27) (Brain-expressed RING finger protein) (RING finger protein 22). Gene symbol: TRIM3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O70277 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ TRIM3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation protein catabolic process at postsynapse (GO:0140252) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Hung AY, et al. "Degradation of postsynaptic scaffold GKAP and regulation of dendritic spine morphology by the TRIM3 ubiquitin ligase in rat hippocampal neurons" PLoS One. 2010 Mar 24;5(3):e9842 PMID:20352094 |
| Figure(s): | Fig.1, 3, 4, 5 |
| Annotation description: | 25/5/2017 Pim TRIM3 was picked up in this paper as a protein with E3-ligase activity (Fig.3) that regulates the protein levels of Shank1A/GKAP (Fig.1). TRIM3 regulates the synaptic (authors have measured GKAP densities/spots around dendritic segments indicative of PSDs localized on dendritic spines) levels of GKAP in a neuronal activity (TTX, bicuculine) dependent manner (Fig.4). Likewise, perturbation of TRIM3 results in change of dendritic spine morphology (GKAP mislocalized and is a scaffolding protein of the PSD; Fig.5). the This could be used for BP annotation against term: 'ubiquitin protein ligase activity (GO:0061630)' regulates 'protein catabolic process (GO:0030163)' occurs_in 'postsynapse (GO:0098794)' From CC annotation in ticket #509 Fig. 2c: biochemical fractionation shows TRIM3 synaptic enrichment. Fig. 2d: TRIM3 is localized at postsynaptic site Litteral: "Subcellular fractionation of rat forebrain showed TRIM3 to be particularly abundant in the light membrane fraction (P3), and sparse in the cytosol (S3) (Figure 2C). TRIM3 was also present in the Triton X-100-resistant PSD fractions (PSDI and PSD II), which corroborates its detection in the PSD by mass spectrometry [22]. However, TRIM3 is not enriched in the PSD like PSD-95 and is extracted by Sarkosyl detergent (PSDIII), indicating that TRIM3 is not tightly associated with the PSD (Figure 2B). By immunostaining, TRIM3 was widely distributed in a micropunctate pattern throughout the cell body, axon, and dendrites of cultured hippocampal neurons (Figure 2C)" |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Chiara Verpelli (ORCID:0000-0003-2949-9725) Carlo Sala (ORCID:0000-0003-0662-9523) |
| Lab: | CNR Neuroscience Institute Milan and Dept. of Biotechnology and Translational Medicine, University of Milan, 20129 Milan, Italy |
| SynGO annotation ID: | 696 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |