| Annotated protein: | Ring finger protein 216. Gene symbol: RNF216. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: D3ZU60 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RNF216 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic endocytic zone (GO:0098843) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Mabb AM, et al. "Triad3A regulates synaptic strength by ubiquitination of Arc" Neuron. 2014 Jun 18;82(6):1299-316 PMID:24945773 |
| Figure(s): | Fig 6 |
| Annotation description: | * GFP-Triad3A colocalized with clathrin-DsRed in dendritic shafts and spines of hippocam- pal neurons (Figure 6A) but not with the postsynaptic density marker Homer-DsRed (Figure 6B). * Endogenous Triad3A in dendritic spines tightly overlapped with clathrin- DsRed within a subdomain of the spine (Figure 6C) * Immunogold electron microscopy (EM) of rat CA1 hippocampus in vivo shows Triad3A immunogold labeling near the membrane and on vesicle-like structures in both presynaptic terminals and postsynaptic spines (Figure 6E). * (>30%) of gold particles were positioned along the plasma membrane near the PSD |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 681 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |