Annotated protein:Dynamin-3 (EC 3.6.5.5). Gene symbol: DNM3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BZ98
antibody wiki:
SynGO gene info:SynGO data @ DNM3
Ontology domain:Biological Process
SynGO term:synaptic vesicle endocytosis (GO:0048488)
Synapse type(s):neocortex
Annotated paper:Raimondi A, et al. "Overlapping role of dynamin isoforms in synaptic vesicle endocytosis" Neuron. 2011 Jun 23;70(6):1100-14 PMID:21689597
Figure(s):fig 4
Annotation description:The authors use dynamin 1 knock out (KO) mice, dynamin 3 KO mice and dynamin 1/3 double KO mice. Using cultured cortical neurons with vGlut1-pHlourin, they demonstrate in fig 4B that fluorescence recovery after stimulation, which is indicative of synaptic vesicle (SV) endocytosis is decreased in dynamin 1 KO mice, is not effected in dynamin 3 KO mice, but is dramatically reduced in dynamin 1/3 double KO mice. Thus, dynamin 1 and 3, which together account for the overwhelming majority of brain dynamin, cooperate in supporting optimal rates of synaptic vesicle endocytosis
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Evidence tracking, Experiment Assay:Optical physiology
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
SynGO annotation ID:676
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology