| Annotated protein: | Dynamin-1 (EC 3.6.5.5). Gene symbol: DNM1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P39053 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DNM1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | neocortex |
| Annotated paper: | Ferguson SM, et al. "A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis" Science. 2007 Apr 27;316(5824):570-4 PMID:17463283 |
| Figure(s): | fig 3, fig 5 |
| Annotation description: | The authors generate dynamin 1 knockout (KO) mice. In fig 3 they demonstrate by electron microscopy that in cortical cultures from these mice, there is an accumulation of clathrin-coated profiles that remain attached to the plasma membrane, clearly indicating a deficiency in the fission of clathrin-coated pits. In fig 5 they use synaptopHlourin (both synaptobrevin and synaptotagmin versions) to measure synaptic vesicle (SV) endocytosis. If one compares fig 5A (no baflomycin) to fig 5B (with baflomycin) one sees that during an intense stimulation (30 secs of 10 Hz stimulus) there is a big difference between wt and KO in terms of the amount of SV recycling. However, at the end of the stimulus (30 sec) the rates of recovery and thus the rates of endocytosis are exactly the same (compare the slopes of the black lines in A versus B). Thus, dynamin 1 is required for SV endocytosis only during active stimulation and not during recovery. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| Additional literature: | Same conclusion with dyn1/3 double KO. @ PMID:21689597 |
| SynGO annotation ID: | 670 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |