Annotated protein:Clathrin heavy chain 1. Gene symbol: CLTC. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P11442
antibody wiki:
SynGO gene info:SynGO data @ CLTC
Ontology domain:Biological Process
SynGO term:synaptic vesicle endocytosis (GO:0048488)
Synapse type(s):hippocampus
Annotated paper:Granseth B, et al. "Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal synapses" Neuron. 2006 Sep 21;51(6):773-86 PMID:16982422
Figure(s):fig 7
Annotation description:The authors use synaptopHlourin (synaptophysin tagged to a pH sensitive GFP) to measure the endocytosis of synaptic vesicles (SVs) into presynaptic nerve terminals in rat hippocampal cultures. Using siRNA to knockdown the annotated protein, clathrin heavy chain (CHC), the authors see reduced SV endocytosis in response to low frequency stimulation.
Evidence tracking, Biological System:Cultured neurons
Evidence tracking, Protein Targeting:RNAi / shRNA
Evidence tracking, Experiment Assay:Optical physiology
Annotator(s):Peter McPherson (ORCID:0000-0001-7806-5662)
Lab:Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
Additional literature:Claims that SV endocytosis is mostly driven by formin in stead of clathrin heavy chain. @ PMID:28231467
SynGO annotation ID:657
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology