| Annotated protein: | Clathrin heavy chain 1. Gene symbol: CLTC. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P11442 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CLTC |
| Ontology domain: | Cellular Component |
| SynGO term: | extrinsic component of synaptic vesicle membrane (GO:0098850) |
| Synapse type(s): | brain |
| Annotated paper: | Maycox PR, et al. "Clathrin-coated vesicles in nervous tissue are involved primarily in synaptic vesicle recycling" J Cell Biol. 1992 Sep;118(6):1379-88 PMID:1325974 |
| Figure(s): | fig 4 and fig 7 |
| Annotation description: | Clathrin-coated vesicles (CCVs), which by nature are coated with the annotated protein, clathrin heavy chain (CHC), are purified from pre-synaptic nerve terminals. The authors demonstrate in figure 2 that the CCVs are from the pre-synaptic compartment because they require osmotic shock to be released (because they are trapped in intact synaptosomes) whereas CCVs from other parts of the cell, including the post-synapse, would not require this treatment. In figure 4 the authors demonstrate by coomassie staining and western blot that the CCVs are highly enriched in CHC and multiple synaptic vesicle (SV) proteins, clearly indicating that CCVs are involved in SV recycling. Figure 7 uses immuno-electron microscopy to demonstrate that CCVs isolated from the pre-synaptic compartment and whole brain show very similar levels of staining for the SV protein synaptophysin, indicating that the major localization of CHC in brain is on CCVs that recycle SVs. 22/5/2017 Pim - I agree with the expert. The vesicles isolated from synaptosomes are (i) shown to contain CHC and (ii) show to be SV's because containing typical SV proteins. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 656 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |