| Annotated protein: | Translational regulator orb2. Gene symbol: ORB2. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: Q9VSR3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPEB2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | brain |
| Annotated paper: | Kruttner S, et al. "Drosophila CPEB Orb2A mediates memory independent of Its RNA-binding domain" Neuron. 2012 Oct 18;76(2):383-95 PMID:23083740 |
| Figure(s): | Fig. 2B |
| Annotation description: | orb2-GFP is detected both pre- and post-synaptically in the mushroom body calyx by immuno-EM using anti-GFP antibodies. In addition, the paper provides evidence of impaired long-term memory in Orb2 mutants, suggesting a role in synaptic plasticity (Fig. 3-4). Both conclusions are supported by Majumdar et al., Cell 2012, and the loss of long.term memory also in Keleman et al., Nat. Neurosci 2007. Note that the fruit fly has only two CPEB isoforms agains four in mammals. Orb2 could equally be the functional ortholog of CPEB2, CPEB3, or CPEB4 (or all three at the same time). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | Orb2 short isoform is prone to prion-like aggregation. The aggregates are also observed in synaptic regions (Fig. 3A); a non-aggregating mutant impairs memory (Fig. 7) @ PMID:22284910 Mutation of Orb2 impairs memory, specifically long-term memory (Fig. 2-3; rescue experiments in later Figures) @ PMID:17965711 |
| SynGO annotation ID: | 655 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |