| Annotated protein: | E3 ubiquitin-protein ligase RNF19A (EC 2.3.2.31) (Double ring-finger protein) (Dorfin) (Gametogenesis-expressed protein GEG-154) (RING finger protein 19A) (UBCM4-interacting protein 117) (UIP117) (XY body protein) (XYbp). Gene symbol: RNF19A. Taxonomy: Mus musculus (Mouse). Uniprot ID: P50636 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RNF19A |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of protein catabolic process at postsynapse, modulating synaptic transmission (GO:0099576) |
| Synapse type(s): | hippocampus, glutamatergic Mossy fiber synapse (DG->CA3) |
| Annotated paper: | Park H, et al. "Mice lacking the PSD-95-interacting E3 ligase, Dorfin/Rnf19a, display reduced adult neurogenesis, enhanced long-term potentiation, and impaired contextual fear conditioning" Sci Rep. 2015 Nov 10;5:16410 PMID:26553645 |
| Figure(s): | Fig 1,4,7 |
| Annotation description: | Dorfin binds to PSD-95 (Figure 1) Dorfin null mutant mice show normal brain and dendrite morphology (Figure 2) Dorfin null mutant mice show increased mEPSC amplitude and enhanced LTP in the hippocampal DG region. mEPSC frequency and paired-pulse ratio is not affected. Neither is mIPSC amplitude/frequency (Figure 4). 23/5/2017 Pim - Dorfin is a known E3 ubiquitin-protein ligase - Fig.7 25 proteins were shown to be under-phosphorylated in hippcampal lysates. - So, dorfin is a known E3 ubiquitin-protein ligase that is active in hippocampus, interacts with PSD-95, and affects postsynaptic, but not presynaptic function of Glutamatergic synapses. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Mass-spectrometry |
| Annotator(s): | Ruud Toonen (ORCID:0000-0002-9900-4233) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 652 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |