| Annotated protein: | Regulating synaptic membrane exocytosis protein 1 (Rab-3-interacting molecule 1) (RIM 1). Gene symbol: RIMS1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9JIR4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RIMS1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone cytoplasmic component (GO:0098831) |
| Synapse type(s): | spinal cord retina |
| Annotated paper: | Wang Y, et al. "Rim is a putative Rab3 effector in regulating synaptic-vesicle fusion" Nature. 1997 Aug 7;388(6642):593-8 PMID:9252191 |
| Figure(s): | fig 3A, 3C |
| Annotation description: | This publication demonstrates with immuno-EM and immunofluorescence that RIM1 is specifically localized at the active zone in spinal cord and ribbon synapses. It makes use of two separate antibodies, plus additional controls (including secondary only). No knockout control is provided. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Wide-field fluorescence |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Shows specificity of the antibody used in this publication with a KO control. @ PMID:27537483 |
| SynGO annotation ID: | 60 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |