| Annotated protein: | Complexin-2 (Complexin II) (CPX II) (Synaphin-1). Gene symbol: CPLX2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P84087 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPLX2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | neocortex cerebellum |
| Annotated paper: | Yamada M, et al. "Immunohistochemical distribution of the two isoforms of synaphin/complexin involved in neurotransmitter release: localization at the distinct central nervous system regions and synaptic types" Neuroscience. 1999;93(1):7-18 PMID:10430466 |
| Figure(s): | Fig.3 |
| Annotation description: | Pre-embedding immuno-EM indicates immunoreactivity for Complexin-2 in dendrites and dendritic spines in different synapse types and brain regions. EM localization indicates that a larger proportion of the protein may be associated to membranes (in comparison to the subcellular fractionation, where most of the synaphins are in the soluble, cytosolic fraction - Referenced to SynGO annotation #577). monoclonal antibodies (mAbs) specific to synaphin 1 (12C5) were generated in the study. No KO control. 31/5/2017 Pim - Authors state: "In the dendrites of immunoreactive neurons, labeling was present along microtubules and the plasma membrane including the postsynaptic densities at the dendritic spines and shafts (Fig. 3B)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 585 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |