| Annotated protein: | Complexin-2 (Complexin II) (CPX II) (Synaphin-1). Gene symbol: CPLX2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P84087 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPLX2 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | neocortex cerebellum |
| Annotated paper: | Yamada M, et al. "Immunohistochemical distribution of the two isoforms of synaphin/complexin involved in neurotransmitter release: localization at the distinct central nervous system regions and synaptic types" Neuroscience. 1999;93(1):7-18 PMID:10430466 |
| Figure(s): | Fig.3 |
| Annotation description: | Pre-embedding immuno-EM indicates immunoreactivity for Complexin-2 in axon terminals (vesicles, plasma membrane) and it filled the axoplasm in a varying degree in different synapse types and brain regions. EM localization indicates that a larger proportion of the protein may be associated to membranes (in comparison to the subcellular fractionation, where most of the synaphins are in the soluble, cytosolic fraction - Referenced to SynGO annotation #577 ). monoclonal antibodies (mAbs) specific to synaphin 1 (12C5) were generated in the study. No KO control. 31/5/2017 Pim - Authors state: "Immunoreactivity is present in many axon terminals (at), in which labeling is associated with synaptic vesicles and the plasma membrane." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | complexin-2[rat: P84087] shown to be mostly present in the cytosplasm as opposed to SV and plasma membrane. [brain/intact tissue/biochem.fract./western blot/antibody] Rationale: Complexin-2 (Synaphin; 19a) in the brain is more concentrated in the soluble fraction (lane 2) than in the insoluble fraction (lane 3) (Fig.4A) and is therefore mainly located in the cytoplasm. "Complexin was scarce in the LP2 fraction (that is rich in synaptic vesicles), indicating that very little, if any, of the protein is associated with synaptic vesicles."(Fig. 5) Complexin in the insoluble fraction is more likely to be on the plasma membranes than on SVs. Antibody not KO-controlled (probably detects Complexin-1 and -2; Fig. 4 B) @ PMID:7654227 |
| SynGO annotation ID: | 582 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |