| Annotated protein: | Complexin-1 (921-S) (Complexin I) (CPX I) (Synaphin-2). Gene symbol: CPLX1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63040 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPLX1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Chang S, et al. "Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse" J Neurosci. 2015 May 27;35(21):8272-90 PMID:26019341 |
| Figure(s): | Fig. 1C, Fig. 11C |
| Annotation description: | Complexin-1 is present in the presynapse (co-localizing with the synaptic vesicle marker Vglut1) in the Calyx of Held terminal of the MNTB in the brainstem (Fig.1C). Complexin-2 expression is downregulated in Calyx of Held terminals during development (Fig.11). At P21, Vglut-1 immunoreactivity does not colocalize with with Complexin-immunoreactivity in Complexin-1 KO mice (Fig.11C). Therefore, the immunoreactivity in wild-type Calyx of Held terminals at P21 must originate from the presence of Complexin-1 in the presynapse (Fig.1C). Complexin-1 immunreactivity is present in the somata of the MNTB principal neurons indicating also a postsynaptic localization. IHC was performed using an antibody (SySy, rabbit anti-Cplx-1/2) that detects Complexin-1 and -2. Complexin-1 KO mice exhibit a loss of immunoreactivity in the somata of the MNTB principal neurons (Fig 11C) indicating the specificity of the antibody. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 581 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |