| Annotated protein: | Complexin-1 (921-S) (Complexin I) (CPX I) (Synaphin-2). Gene symbol: CPLX1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63040 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPLX1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of exocytic insertion of neurotransmitter receptor to postsynaptic membrane (GO:0099145) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Ahmad M, et al. "Postsynaptic complexin controls AMPA receptor exocytosis during LTP" Neuron. 2012 Jan 26;73(2):260-7 PMID:22284181 |
| Figure(s): | Fig. 1, Fig. 2, Fig.4 |
| Annotation description: | Postsynaptic knock-down (KD) of Complexin-1 and -2 (shRNA stereotactic in vivo injection into CA1 region of the hippocampus) blocks LTP (Fig. 1 F), indicating that Complexin plays a role in postsynaptic membrane fusion events underlying the exocytosis of AMPA receptors during LTP. NMDA-Receptor mediated LTD is unaffected by postsynaptic Complexin KD (Fig. 1 H). Block of LTP can be rescued by expression of a shRNA-resitant full-length Complexin-1 variant (Fig. 1 G). Basal synaptic transmission is not affected by postsynaptic Complexin KD in slices (Fig.2) and cultures (Fig 4B), indicating that Complexin only regulates AMPA-Receptor exocytosis. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 578 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |