| Annotated protein: | Complexin-1 (921-S) (Complexin I) (CPX I) (Synaphin-2). Gene symbol: CPLX1. Taxonomy: Mus musculus (Mouse). Uniprot ID: P63040 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CPLX1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle fusion to presynaptic active zone membrane (GO:0031630) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Reim K, et al. "Complexins regulate a late step in Ca2+-dependent neurotransmitter release" Cell. 2001 Jan 12;104(1):71-81 PMID:11163241 |
| Figure(s): | Fig. 2, Table 1, Fig.5 |
| Annotation description: | Single, autaptic hippocampal Complexin-1 and -2 double knock-out (DKO) neurons exhibit a decrease in evoked release (EPSC amplitude; Fig. 2A, B; Table 1), but not in the readily-relasable pool size (sucrose response; Fig 2D, E; Table 1). Complexin-1 and -2 can compensate for the loss of the respective other isoform in hippocampal autaptic neurons: Complexin-1 KO mice and Complexin-2 KO mice are viable and exhibit no deficits in evoked release (Fig 2B), whereas Complexin-1/2 DKO mice die a few hours after birth. Complexin only regulates synaptic vesicle fusion, since the deficit in synaptic transmission can be rescued by elevating the extracellular calcium concentration (Fig. 5). |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 575 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |