| Annotated protein: | Actin-binding protein WASF1 (Protein WAVE-1) (Wiskott-Aldrich syndrome protein family member 1) (WASP family protein member 1). Gene symbol: WASF1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R5H6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ WASF1 |
| Ontology domain: | Biological Process |
| SynGO term: | modification of postsynaptic actin cytoskeleton (GO:0098885) |
| Synapse type(s): | striatum hippocampus |
| Annotated paper: | Kim Y, et al. "Phosphorylation of WAVE1 regulates actin polymerization and dendritic spine morphology" Nature. 2006 Aug 17;442(7104):814-7 PMID:16862120 |
| Figure(s): | fig 1-3 |
| Annotation description: | Spine morphology of medium spiny neurons in striatal sections obtained from WAVE1-/- mice revealed that WAVE1-/- neurons displayed a significant reduction in class 1 spines (stubby type), class 2 spines (comprised of mature spines with a relatively large head and a short neck) and class 3 spines (thin spines) and showed a relatively high density of class 4 protrusions, or filopodia. Furthermore WAVE1 RNAi in primary hippocampal neurons resulted in a decrease in the density of class 2 spines and an increase in filopodia. 30/8/2017 Pim - Fig.1 WAVE1 co-ips with actin - Fig.2 Inhibition of Arp2/3-complex-mediated actin polymerization by phosphorylation of WAVE1. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Cell-free system |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Alexandros Kanellopoulos (ORCID:0000-0002-2094-7491) Vittoria Mariano (ORCID:0000-0002-9848-0262) Achsel Tilmann (ORCID:0000-0002-1190-4481) Claudia Bagni (ORCID:0000-0002-4419-210X) |
| Lab: | Department of Fundamental Neurosciences, University of Lausanne, CH-1006 Lausanne, Switzerland; Dept Biomedicine and Prevention, University of Rome Tor Vergata, 00133 Rome, Italy |
| Additional literature: | Chronic cocaine-induced changes in dendritic spine density in slices from WT mice and WAVE1 D1-KO mice. In response to cocaine a comparable increase in the density of total spines and class 2 spines in the NAc core of WT or WAVE1 D1-KO mice (Fig. 4) @ PMID:19460862 |
| SynGO annotation ID: | 571 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |