| Annotated protein: | Potassium channel tetramerization domain containing 16. Gene symbol: KCTD16. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: A0A8I6A6D0 |
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| SynGO gene info: | SynGO data @ KCTD16 |
| Ontology domain: | Biological Process |
| SynGO term: | G protein-coupled neurotransmitter receptor activity involved in regulation of presynaptic membrane potential (GO:0150047) |
| Annotated paper: | Schwenk J, et al. "Native GABA(B) receptors are heteromultimers with a family of auxiliary subunits" Nature. 2010 May 13;465(7295):231-5 PMID:20400944 |
| Figure(s): | Figure 1, 3, 4 |
| Annotation description: | Figure 1: identification of KCTD8, KCTD12, KCTD12B and KCTD16 as subunits of native GABAB receptors by AP+ MS of both GABAB1 and GABAB2 receptors. No KCTD-specific peptides were detected in affinity purifications with anti-GABAB1 and anti-GABAB2 from brains of GABAB1 and GABAB2 knockout mice, respectively. Co-assembly of the identified KCTD proteins with native GABAB receptors was further corroborated by reverse affinity purifications (Supplementary Fig. 3C + 6) and western blot analysis of native gel separations of brain membrane fractions (Fig. 1C) Figure 4b: Experiments in CHO cells, co-expressing co-expressing GABAB, Kir3.1/3.2 channels and the different KCTD proteins. GABAB-activated Kir3 currents elicited by 25-s applications of baclofen the pronounced differences in kinetics upon expression of KCTD16 compared to without. Figure 4c: KCDT16 significantly influenced the agonist concentration dependence of the GABAB responses as determined by apparent dose- response relationships with baclofen. Taken together, the paper demonstrates that KCTD proteins endow GABAB receptors with subtype-specific properties of their G-protein signalling, regulating the the activity of voltage-gated calcium and inward-rectifier potassium channels. As KCTD16 GABAB receptors, Kir3 channels and CaV channels all localize to both the pre- and postsynaptic membrane, the GO-term "G protein-coupled neurotransmitter receptor activity involved in regulation of presynaptic membrane potential" was selected, as well as "G protein-coupled neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential" (annotation #5556) |
| Evidence tracking, Biological System: | Cultured neurons Non-neuronal tissue Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electrophysiology (generic) IP + WB/MSMS |
| Annotator(s): | Robin Clement Guus Smit Matthijs Verhage |
| ORCID: | 0000-0002-2286-1587 0000-0002-2514-0216 |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5575 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |