| Annotated protein: | Vacuolar protein sorting-associated protein 26B (Vesicle protein sorting 26B). Gene symbol: VPS26B. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8C0E2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS26B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic early endosome (GO:0098842) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Simoes S, et al. "Alzheimer's vulnerable brain region relies on a distinct retromer core dedicated to endosomal recycling" Cell Rep. 2021 Dec 28;37(13):110182 PMID:34965419 |
| Figure(s): | Fig 2, 4C, S3 |
| Annotation description: | Fig 4C shows that reexpression of GFP-VPS26B in VPS26B knock-out neurons the GFP signal overlaps with membrane GluA1 staining, indicating that VPS26B can be localized at (post)synaptic sites. Fig 2A, C shows colocalization of endogenous VPS26B with somatodendritic early endosome marker EEA1 and recycling endosome markers Syntaxin13 and pulse-chase transferrin. Fig 2B and Fig S3 confirm these results at the ultrastructural level using immunogold immuno-EM |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Electron Microscopy |
| Annotator(s): | Jan van Weering (ORCID:0000-0001-5259-4945) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5551 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |