| Annotated protein: | Protein unc-13 homolog A (Munc13-1). Gene symbol: UNC13A. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q4KUS2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UNC13A |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone (GO:0048786) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Chen Z, et al. "The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse" J Neurosci. 2013 May 8;33(19):8336-51 PMID:23658173 |
| Figure(s): | Fig. 1-3 and Table 1 |
| Annotation description: | Localization of Munc13 isoforms at the calyx of Held synapse. - Fig.1 shows localization of native protein with subtype-specific antibodies. Strong colocalization with the presynaptic marker VGlut1 together with a lack of staining of the postsynaptic MNTB cell body strongly support a presynaptic localization. - Fig.2/3 shows localization of genetically encoded EGFP attached to native mouse Munc13 paralogs. Strong colocalization with the presynaptic markers VGlut1 and Bassoon together with a lack of staining of the postsynaptic MNTB cell body strongly support a presynaptic localization. Table 1 presents a quantification of Munc13-1 staining intensity at Bassoon-immunoreactive active zones, demonstrating a high degree of localization to the presynaptic active zone. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 555 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |