| Annotated protein: | Guanine deaminase (Guanase) (Guanine aminase) (EC 3.5.4.3) (Guanine aminohydrolase) (GAH). Gene symbol: GDA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9WTT6 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GDA |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic cytosol (GO:0099524) |
| Annotated paper: | Firestein BL, et al. "Cypin: a cytosolic regulator of PSD-95 postsynaptic targeting" Neuron. 1999 Nov;24(3):659-72 PMID:10595517 |
| Figure(s): | Figures 3, 4, 5 |
| Annotation description: | Figure 1: characterization of the Cypin protein. The peptide sequence shown here is a 100% match to the protein GDA (Guanine deaminase, p51-nedasin). Figure 3: GDA/Cypin Interacts with PDZ1 and PDZ2 of PSD-95 through a C-Terminal -SSSV* Consensus Sequence Figure 4: GDA/Cypin is co-enriched with DLG1-4 in synaptic membrane fractions prepared from rat brain. Figure 5: GDA/Cypin localizes to both pre- and postsynaptic sites in cortical neurons, as shown by immuno-EM. "Though PSDs are occasionally coated with the peroxidase label, cypin immunoreactivity often occurs only in the dendritic shaft and neck of the spine and is typically excluded from the spine head and PSD (Figure 6A) ... Within axons, cypin is concentrated at the synaptic terminals, and the reaction product is often seen coating synaptic vesicles (Figure 6C). This is consistent with subcellular fractionation, which shows that cypin is expressed both pre- and postsynaptically." |
| Evidence tracking, Biological System: | Intact tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Antibody (detection) Over-expression |
| Evidence tracking, Experiment Assay: | IP + WB/MSMS Y2H Electron Microscopy |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5549 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |