| Annotated protein: | Actin-binding LIM protein 1 (abLIM-1) (Actin-binding LIM protein family member 1). Gene symbol: ABLIM1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8K4G5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABLIM1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | forebrain |
| Annotated paper: | Fernandez E, et al. "Targeted tandem affinity purification of PSD-95 recovers core postsynaptic complexes and schizophrenia susceptibility proteins" Mol Syst Biol. 2009;5:269 PMID:19455133 |
| Figure(s): | Table 1, Figure 4 |
| Annotation description: | Ablim1 (Actin-binding LIM protein 1) was identified as a PSD95 (DLG4, here the bait protein) interaction partner through tandem affinity purification (TAP) proteomics (Fig. 3, Table 1). This was validated by western blot; pulldown of endogenous Ablim1 using an antibody yields PSD95. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot IP + WB/MSMS |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| Additional literature: | Synapse-enriched fractions (P2) were prepared from mouse striatum. Ablim1 was identified by mass-spectrometry (Fig. 1) and western blot validations (Fig. 2) @ PMID:22952455 "The abLIM1 is a nonerythroid actin-binding protein critical for stable plasma membrane-cortex interactions under mechanical tension. ... Our results suggest that abLIM1 functions as an LLPS-dependent actin nucleator and cross-linker and provide insights into how LLPS-induced condensates could self-construct intracellular architectures of high connectivity and plasticity." @ PMID:35858327 Ablim1 is highly enriched in PSD biochemical fractions as compared to various other synaptic fractions @ PMID:28935861 |
| SynGO annotation ID: | 5547 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |