| Annotated protein: | Ubiquitin-protein ligase E3B (EC 2.3.2.26) (HECT-type ubiquitin transferase E3B). Gene symbol: UBE3B. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9ES34 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ UBE3B |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | cerebral cortex, glutamatergic hippocampus, glutamatergic |
| Annotated paper: | Ambrozkiewicz MC, et al. "The murine ortholog of Kaufman oculocerebrofacial syndrome protein Ube3b regulates synapse number by ubiquitinating Ppp3cc" Mol Psychiatry. 2021 Jun;26(6):1980-1995 PMID:32249816 |
| Figure(s): | Figure 1d, 3 |
| Annotation description: | Figure 1D: Ube3b is enriched in PSD and crude synaptic membrane biochemical fractions, similar to PSD95 (DLG4), prepared from cerebral cortices from 6- to 7-week-old mice. Fig. 3: Ube3b knockout leads to increased density and altered spine morphology in hippocampal neurons. Necks of spines in Ube3b KO hippocampal neurons were longer (Fig. 3c, g and Table S1L), and spine heads wider as compared with control (Fig. 3c, h and Table S1M). Fig. 4: Ube3b knockout leads to an increase in the number of functional excitatory synapses in hippocampal neurons. Fig. 6: Proteomic screen identifies Ppp3cc as a Ube3b substrate. Ppp3cc overexpression phenocopies Ube3b cKO in spine formation in vivo. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5544 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |