| Annotated protein: | Misshapen-like kinase 1 (EC 2.7.11.1) (GCK family kinase MiNK) (MAPK/ERK kinase kinase kinase 6) (MEK kinase kinase 6) (MEKKK 6) (Misshapen/NIK-related kinase) (Mitogen-activated protein kinase kinase kinase kinase 6). Gene symbol: MINK1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1LP90 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MINK1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse organization (GO:0099175) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Hussain NK, et al. "MINK and TNIK differentially act on Rap2-mediated signal transduction to regulate neuronal structure and AMPA receptor function" J Neurosci. 2010 Nov 3;30(44):14786-94 PMID:21048137 |
| Figure(s): | Figure 2, 3 |
| Annotation description: | Figure 1C: MINK and TNIK enriched in PSD biochemical fractions prepared from rat brain, as compared to S1, S2, P1, P2 fractions. Figure 1D: In cultured hippocampal neurons at DIV23, a polyclonal antibody against MINK/TNIK showed localization to synaptic puncta (co-staining with Bassoon and Phalloidin, resolution does not allow for distinction between pre/post-synapse). Figure 2C: RNAi knockdown of MINK in hippocampal cultures after 18 DIV significantly reduced dendritic arbor complexity relative to control neurons. Figure 3: MINK and TNIK knockdown modulate spine density and surface expression of AMPA receptors. Figure 4: MINK and TNIK localization in neurons is modulated by the activation state of Rap2. Figure 6: MINK blocks Rap2-mediated reduction in surface GluR1 clusters. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5543 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |