| Annotated protein: | Epidermal growth factor receptor substrate 15-like 1 (Epidermal growth factor receptor pathway substrate 15-related sequence) (Eps15-rs) (Eps15-related protein) (Eps15R). Gene symbol: EPS15L1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q60902 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPS15L1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Milesi C, et al. "Redundant and nonredundant organismal functions of EPS15 and EPS15L1" Life Sci Alliance. 2019 Jan 28;2(1):e201800273 PMID:30692166 |
| Figure(s): | Figure 2, S2 |
| Annotation description: | Figure S2: "By immunofluorescence analysis of the hippocampus in adult mouse brain sections, both EPS15 and EPS15L1 localized to neurons, and EPS15 also showed substantial staining in cells with astrocytic morphology (Fig S2C). Of interest, EPS15L1 showed co-localization with synaptophysin, a presynaptic marker, whereas EPS15 did not (Fig S2C). This was further corroborated by a biochemical fractionation of adult mouse brain in which we compared the total homogenate (H) to the synaptosomal fraction and to the postsynaptic density. Of the several endocytic proteins tested, EPS15L1 was the sole one clearly enriched in the synaptosomal fraction (marginal enrichment was also detected for dynamin and AP2; Fig S2D)." Figure 2: "thus, we proceeded with an ultrastructural analysis of Eps15L1-KO neurons. We detected a reduction of synaptic vesicles in Eps15L1-KO synapses of about 50%, although synapses from Eps15-KO were comparable with WT (Fig 2D). The number of docked/ tethered vesicles was also significantly decreased in Eps15L1-KO synapses (Fig 2E)." "To test whether the absence of evident phenotypes in the dye uptake assay was due to the up-regulation of compensatory bulk endocytosis, we measured by EM the number of vesicles with a diameter higher than 80 nm, as bulk endocytosis is typically characterized by large invaginations of the plasma membrane which then fission to form endosomal-like compartments (Cousin, 2009; Saheki & De Camilli, 2012). Under steady state conditions, we did not observe differences in the number of this type of vesicles (Fig 2F). However, when we followed HRP uptake upon depolarization with 50 mM KCl (Fig 2G, left), we observed a significant increase in large HRP-positive structures in Eps15L1-KO neurons (Fig 2G, center and right), suggesting that bulk endocytosis is indeed more active in these cells." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) Confocal |
| Annotator(s): | Frank Koopmans (ORCID:0000-0002-4973-5732) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 5538 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |